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When was penicillin first used as a practical antibiotic?

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The action of penicillin was first observed by Sir Alexander Flemming in 1928. It was developed as a practical antibiotic during the late '30's and early '40's, becoming available in quantities in time to treat the Allied wounded on D-Day. About.com has a nice write-up:

http://inventors.about.com/library/inventors/blpenicillin.htm

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